Patch clamp myocardial cell current and voltage recording (Action potential) technology platform
Release time:
2025-07-02 16:11
Source:
Patch clamp myocardial cell current and voltage recording
(Action potential) technology platform
1. Representative curves of current and voltage in rat cardiomyocytes:
2. Instrument: Patch clamp amplifier 200B (Axon, USA).
Patch clamp and calcium release combination device.
3. Papers published on this platform:
(1) Long Chen, et al. Confi rmation of a proarrhythmic risk underlying the clinical use of common Chinese herbal intravenous injections. J of Ethnopharmacology. 2012; 142:829-835.
(2) Wang YW, et al. Bay 60-7550, a PDE2 inhibitor, exerts positive inotropic effect of rat heart by increasing PKA-mediated phosphorylation of phospholamban. Eur J Pharmacol. 2021; 901:174077.
(3) Huang H, et al. Rolipram, a PDE4 Inhibitor, Enhances the Inotropic Effect of Rat Heart by Activating SERCA2a. Front. Pharmacol. 2019, 10:221.
(4) Xie M, et al. Increased sarcoplasmic/endoplasmic reticulum calcium ATPase 2a activity underlies the mechanism of the positive inotropic effect of ivabradine. Exp Physiol. 2020; 105(3):477-488.
Link to paper:
https://www.sciencedirect.com/science/article/abs/pii/S0378874112003959
https://www.sciencedirect.com/science/article/abs/pii/S0014299921002302
http://www.ncbi.nlm.nih.gov/pubmed/30967774
https://physoc.onlinelibrary.wiley.com/doi/abs/10.1113/EP087964
4. Application fields:
This technology has the widest application and can be used to study any cell with membrane channels, including myocardial cells, nerve cells, renal tissue cells, tumor cells, immune cells, etc. It can analyze the mechanisms of action of all bioactive substances and drugs related to ion channels; Perform functional analysis of protein translation after knocking out genes associated with ion channels.
5. Technical support:
E-mail:longchen@njucm.edu.cn; Cellular phone: 13584058521
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Add: 8th Floor, Block D, No. 8 Taohongjing Road, Medical High-tech Zone, Taizhou City, Jiangsu
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