Rat isolated heart technology platform
Release time:
2025-07-02 16:10
Source:
Rat isolated heart technology platform
1. Indicators and representative curves for isolated heart studies:
(1) The indicators of isolated heart electrocardiogram: heart rate (HR); P-R interval; QT interval (QTc) (QTc=QT × 10001/3/RR1/3).
This figure illustrates the representative curve of the effect of Quinidine on the electrocardiogram of isolated rat hearts.
(2) The indicators of isolated heart cardiac contractility include heart rate, systolic blood pressure, diastolic blood pressure, maximum rate of increase in left ventricular pressure, and developing blood pressure.
This figure illustrates the effect of phosphodiesterase type 4 (PDE4) inhibitor (Rolipram) on isolated heart cardiac contractility. (A) For autonomous heart rate, (B)The change in contractility under electrical stimulation conditions (4 beats per second, 4Hz).
2. Instrument: RM-6240D four channel physiological recorder and supporting recording analysis software (Chengdu Instrument Factory).
Isolated heart cardiac perfusion platform
3. Papers published on this platform:
(1) Gao L, et al. Novel in vivo and in vitro mechanisms of positive inotropic effect of atractylodin. Clin Exp Pharmacol Physiol. 2020 Sep 15. doi: 10.1111/1440-1681.13406.
(2) Xue Shuyin et al.: Comparison of the hemodynamic effects of deacetylated verbascoside and milrinone in rats. Chinese Pharmacology and Journal of Toxicology 2017; 31(2): 179-186. P109
(3) Zhu Xiaojia et al.: Positive inotropic effect and mechanism of phosphodiesterase type 9 inhibitor PF-04449613 on rat heart. Journal of Physiology, 2021, 73 (2): 275-285. P136
Link to paper:
https://onlinelibrary.wiley.com/doi/epdf/10.1111/1440-1681.13406
https://cdmd.cnki.com.cn/Article/CDMD-10315-1018133680.htm
https://www.nstl.gov.cn/paper_detail.html?id=af83bde8cc4772fed532a972380cfaba
4. Application fields:
The isolated heart is an indispensable method for analyzing the direct effects of bioactive substances on the heart under the condition of removing neurohumoral regulation, and for studying the mechanism of drug direct effects on the heart. It is also an important means of analyzing whether drugs exert their effects through metabolic pathways or directly. It is an indispensable technique for the analysis of drug cardiac safety and the study of positive muscle strength mechanisms.
5. Technical support:
E-mail:longchen@njucm.edu.cn; Cellular phone: 13584058521
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Add: 8th Floor, Block D, No. 8 Taohongjing Road, Medical High-tech Zone, Taizhou City, Jiangsu
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